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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

RABL2A Polyclonal antibody

RABL2A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, RIP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17816-1-AP

Synonyms

Rab like protein 2A, RABL2A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RABL2A Polyclonal antibody (17816-1-AP)

    WB analysis using 17816-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17816-1-AP (RABL2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RABL2A Polyclonal antibody (17816-1-AP)

    WB analysis of human brain using 17816-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17816-1-AP (RABL2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using RABL2A Polyclonal antibody (17816-1-AP)

    IHC staining of mouse ovary using 17816-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17816-1-AP (RABL2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using RABL2A Polyclonal antibody (17816-1-AP)

    IHC staining of mouse ovary using 17816-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17816-1-AP (RABL2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RABL2A Polyclonal antibody (17816-1-AP)

    IHC staining of human ovary tumor using 17816-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17816-1-AP (RABL2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RABL2A Polyclonal antibody (17816-1-AP)

    IHC staining of human ovary tumor using 17816-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17816-1-AP (RABL2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse pancreas tissue, human brain tissue, mouse brain tissue
Positive IHC detected inmouse ovary tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17816-1-AP targets RABL2A in WB, IP, RIP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABL2A fusion protein Ag12135 相同性解析による交差性が予測される生物種
Full Name RAB, member of RAS oncogene family-like 2A
Calculated molecular weight165aa,18 kDa; 228aa,26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC111008
Gene symbol RABL2A
Gene ID (NCBI) 11159
RRIDAB_2175953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RABL2A antibody 17816-1-APDownload protocol
IHC protocol for RABL2A antibody 17816-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

DNA Cell Biol

RABL2A-CCDC34 Axis Promotes Sorafenib Resistance in Hepatocellular Carcinoma.

Authors - Mingxiu Zhou
  • KD Validated
View Article
humanWB,RIP

RNA Biol

SNHG15 aids SARS-CoV-2 entry via RABL2A

Authors - Samuel Pushparaj
  • KD Validated
View Article