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Host
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RABL2B Recombinant antibody, PBS Only

RABL2B Recombinant Antibody for ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA, WB

Conjugate

Unconjugated

CloneNo.

240501D3

Cat no : 83470-3-PBS

Synonyms

Rab-like protein 2B, Rab like protein 2B, 240501D3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RABL2B Recombinant antibody (83470-3-RR)

    WB analysis using 83470-3-RR (same clone as 83470-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83470-3-RR (RABL2B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83470-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using RABL2B Recombinant antibody (83470-3-RR)

    FC experiment of A549 using 83470-3-RR (same clone as 83470-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83470-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using RABL2B Recombinant antibody (83470-3-RR)

    FC experiment of MCF-7 using 83470-3-RR (same clone as 83470-3-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83470-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83470-3-RR

    Affinity and Kinetic Characterization of 83470-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83470-3-RR against Human RABL2B were performed. The affinity constant is 0.256 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83470-3-PBS is RABL2B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RABL2B fusion protein Ag2189 相同性解析による交差性が予測される生物種
Full Name RAB, member of RAS oncogene family-like 2B
Calculated molecular weight 229 aa, 26 kDa
GenBank accession numberBC024281
Gene symbol RABL2B
Gene ID (NCBI) 11158
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.