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KD/KO Validated

RABL3 Polyclonal antibody

Name: RABL3 Polyclonal antibody
Brand: Proteintech
SKU: 11916-1-AP

RABL3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11916-1-AP

Synonyms

Rab-like protein 3, Rab like protein 3

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis of human lung using 11916-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 11916-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11916-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11916-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11916-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 11916-1-AP
IP result of anti-RABL3 (IP:11916-1-AP, 3ug; Detection:11916-1-AP 1:300) with mouse lung tissue lysate 4000ug.
IHC staining of human breast cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cervical cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11916-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A549 using 11916-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RABL3 antibody (11916-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HEK-293 using 11916-1-AP
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human RABL3 (11916-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 11916-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, mouse brain tissue, human brain tissue, human lung tissue, HUVEC cells, U-251 cells
Positive IP detected in	mouse lung tissue
Positive IHC detected in	human breast cancer tissue, human liver cancer tissue, human cervical cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells
Positive FC (Intra) detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 2 publications below

Product Information

11916-1-AP targets RABL3 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RABL3 fusion protein Ag2531 相同性解析による交差性が予測される生物種
Full Name	RAB, member of RAS oncogene family-like 3
Calculated molecular weight	236 aa, 26 kDa
Observed molecular weight	26 kDa
GenBank accession number	BC020832
Gene symbol	RABL3
Gene ID (NCBI)	285282
RRID	AB_2175960
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The gene of Rabl3 is located on 3q13.3 and is composed of eight exons and seven introns. It is a member of the Rab subfamily, the largest group in the Ras superfamily of small guanosine triphosphatases (GTPases).

Protocols

Product Specific Protocols
WB protocol for RABL3 antibody 11916-1-AP	Download protocol
IHC protocol for RABL3 antibody 11916-1-AP	Download protocol
IF protocol for RABL3 antibody 11916-1-AP	Download protocol
IP protocol for RABL3 antibody 11916-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncol Rep Evaluation of the novel gene Rabl3 in the regulation of proliferation and motility in human cancer cells. Authors - Li Qi Q KD Validated View Article
human	WB	Med Sci Monit High Expression of Rab-like 3 (Rabl3) is Associated with Poor Survival of Patients with Non-Small Cell Lung Cancer via Repression of MAPK8/9/10-Mediated Autophagy. Authors - Weihua Zhang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RABL3 Polyclonal antibody

RABL3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis of human lung using 11916-1-AP

WB analysis using 11916-1-AP

WB analysis of human brain using 11916-1-AP

WB analysis of HEK-293 using 11916-1-AP

WB analysis of mouse brain using 11916-1-AP

IP experiment of mouse lung using 11916-1-AP

IHC staining of human breast cancer using 11916-1-AP

IHC staining of human breast cancer using 11916-1-AP

IHC staining of human liver cancer using 11916-1-AP

IHC staining of human liver cancer using 11916-1-AP

IHC staining of human breast cancer using 11916-1-AP

IHC staining of human breast cancer using 11916-1-AP

IHC staining of human cervical cancer using 11916-1-AP

IHC staining of human cervical cancer using 11916-1-AP

IHC staining of human kidney using 11916-1-AP

IHC staining of human kidney using 11916-1-AP

IHC staining of human kidney using 11916-1-AP

IHC staining of human kidney using 11916-1-AP

IF Staining of A549 using 11916-1-AP

FC experiment of HEK-293 using 11916-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Evaluation of the novel gene Rabl3 in the regulation of proliferation and motility in human cancer cells.

High Expression of Rab-like 3 (Rabl3) is Associated with Poor Survival of Patients with Non-Small Cell Lung Cancer via Repression of MAPK8/9/10-Mediated Autophagy.