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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RABL3 Monoclonal antibody

RABL3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H3E1

Cat no : 68342-1-Ig

Synonyms

Rab like protein 3, RABL3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RABL3 Monoclonal antibody (68342-1-Ig)

    WB analysis using 68342-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68342-1-Ig (RABL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RABL3 Monoclonal antibody (68342-1-Ig)

    IF Staining of A549 using 68342-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RABL3 antibody (68342-1-Ig, Clone: 2H3E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68342-1-Ig targets RABL3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RABL3 fusion protein Ag33137 相同性解析による交差性が予測される生物種
Full Name RAB, member of RAS oncogene family-like 3
Calculated molecular weight 236 aa, 26 kDa
Observed molecular weight26 kDa
GenBank accession numberBC020832
Gene symbol RABL3
Gene ID (NCBI) 285282
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The gene of Rabl3 is located on 3q13.3 and is composed of eight exons and seven introns. It is a member of the Rab subfamily, the largest group in the Ras superfamily of small guanosine triphosphatases (GTPases).

Protocols

Product Specific Protocols
WB protocol for RABL3 antibody 68342-1-IgDownload protocol
IF protocol for RABL3 antibody 68342-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols