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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFT22 Polyclonal antibody

IFT22 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26896-1-AP

Synonyms

IFT22, Rab like protein 5, RABL5

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IFT22 Polyclonal antibody (26896-1-AP)

    WB analysis of HeLa using 26896-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26896-1-AP (IFT22 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using IFT22 Polyclonal antibody (26896-1-AP)

    WB analysis of mouse brain using 26896-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26896-1-AP (IFT22 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using IFT22 Polyclonal antibody (26896-1-AP)

    IHC staining of human colon using 26896-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26896-1-AP (IFT22 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using IFT22 Polyclonal antibody (26896-1-AP)

    IHC staining of human colon using 26896-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26896-1-AP (IFT22 antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26896-1-AP is IFT22 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT22 fusion protein Ag25292 相同性解析による交差性が予測される生物種
Full Name RAB, member RAS oncogene family-like 5
Observed molecular weight 21 kDa
GenBank accession numberBC038668
Gene symbol IFT22
Gene ID (NCBI) 64792
RRIDAB_2880672
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IFT22 antibody 26896-1-APDownload protocol
IHC protocol for IFT22 antibody 26896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols