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  • Featured Product
  • KD/KO Validated

RAC1 Monoclonal antibody, PBS Only

RAC1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

4A4B11

Cat no : 66122-1-PBS

Synonyms

MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAC1 Monoclonal antibody (66122-1-Ig)

    WB analysis using 66122-1-Ig (same clone as 66122-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66122-1-Ig (Rac1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using RAC1 Monoclonal antibody (66122-1-Ig)

    WB analysis of NIH/3T3 using 66122-1-Ig (same clone as 66122-1-PBS)

    WB result of RAC1 antibody (66122-1-Ig, 1:2000) with si-control and si-RAC1 transfected NIH3T3 cells. This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RAC1 Monoclonal antibody (66122-1-Ig)

    WB analysis of HeLa using 66122-1-Ig (same clone as 66122-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66122-1-Ig (Rac1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using RAC1 Monoclonal antibody (66122-1-Ig)

    WB analysis of human brain using 66122-1-Ig (same clone as 66122-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66122-1-Ig (RAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HL-60 cells using RAC1 Monoclonal antibody (66122-1-Ig)

    IP experiment of HL-60 using 66122-1-Ig (same clone as 66122-1-PBS)

    IP result of anti-RAC1 (IP:66122-1-Ig, 3ug; Detection:66122-1-Ig 1:700) with HL-60 cells lysate 4000ug. This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAC1 Monoclonal antibody (66122-1-Ig)

    IHC staining of human colon cancer using 66122-1-Ig (same clone as 66122-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66122-1-Ig (RAC1 Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAC1 Monoclonal antibody (66122-1-Ig)

    IHC staining of human colon cancer using 66122-1-Ig (same clone as 66122-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66122-1-Ig (RAC1 Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RAC1 Monoclonal antibody (66122-1-Ig)

    IF Staining of NIH/3T3 using 66122-1-Ig (same clone as 66122-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed NIH/3T3 cells using 66122-1-Ig(Rac1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAC1 Monoclonal antibody (66122-1-Ig)

    IF Staining of HeLa using 66122-1-Ig (same clone as 66122-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66122-1-Ig(Rac1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66122-1-PBS targets RAC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RAC1 fusion protein Ag17779 相同性解析による交差性が予測される生物種
Full Name ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC004247
Gene symbol RAC1
Gene ID (NCBI) 5879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.