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  • Featured Product
  • KD/KO Validated

RAC2 Monoclonal antibody, PBS Only

RAC2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4C2H6

Cat no : 60077-1-PBS

Synonyms

Ras-related C3 botulinum toxin substrate 2, p21-Rac2, p21 Rac2, HSPC022, Gx

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAC2 Monoclonal antibody (60077-1-Ig)

    WB analysis using 60077-1-Ig (same clone as 60077-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60077-1-Ig (RAC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60077-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using RAC2 Monoclonal antibody (60077-1-Ig)

    IHC staining of human tonsillitis using 60077-1-Ig (same clone as 60077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60077-1-Ig (RAC2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60077-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using RAC2 Monoclonal antibody (60077-1-Ig)

    IHC staining of human tonsillitis using 60077-1-Ig (same clone as 60077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60077-1-Ig (RAC2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60077-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using RAC2 Monoclonal antibody (60077-1-Ig)

    IHC staining of human tonsillitis using 60077-1-Ig (same clone as 60077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60077-1-Ig (RAC2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60077-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using RAC2 Monoclonal antibody (60077-1-Ig)

    IHC staining of human tonsillitis using 60077-1-Ig (same clone as 60077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60077-1-Ig (RAC2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60077-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60077-1-PBS targets RAC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAC2 fusion protein Ag1202 相同性解析による交差性が予測される生物種
Full Name ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC001485
Gene symbol RAC2
Gene ID (NCBI) 5880
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RAC2, also named as GX, belongs to the small GTPase superfamily and Rho family. RAC2 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 augments the production of reactive oxygen species (ROS) by NADPH oxidase. Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome.