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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RACGAP1 Polyclonal antibody

RACGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30042-1-AP

Synonyms

HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RACGAP1 Polyclonal antibody (30042-1-AP)

    WB analysis using 30042-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30042-1-AP (RACGAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RACGAP1 Polyclonal antibody (30042-1-AP)

    IHC staining of mouse testis using 30042-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30042-1-AP (RACGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RACGAP1 Polyclonal antibody (30042-1-AP)

    IHC staining of mouse testis using 30042-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30042-1-AP (RACGAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RACGAP1 Polyclonal antibody (30042-1-AP)

    IF Staining of MCF-7 using 30042-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RACGAP1 antibody (30042-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30042-1-AP targets RACGAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RACGAP1 fusion protein Ag32468 相同性解析による交差性が予測される生物種
Full Name Rac GTPase activating protein 1
Calculated molecular weight 632 aa, 71 kDa
Observed molecular weight71 kDa
GenBank accession numberBC032754
Gene symbol RACGAP1
Gene ID (NCBI) 29127
RRIDAB_3086216
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RACGAP1 antibody 30042-1-APDownload protocol
IHC protocol for RACGAP1 antibody 30042-1-APDownload protocol
IF protocol for RACGAP1 antibody 30042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols