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KD/KO Validated

RACK1; GNB2L1 Polyclonal antibody

Name: RACK1; GNB2L1 Polyclonal antibody
Brand: Proteintech
SKU: 27592-1-AP

RACK1; GNB2L1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (3)

Applications

WB, IP, IF, RIP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 27592-1-AP

Synonyms

Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1, RACK1; GNB2L1

Validation Data Gallery

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AllWBIHC

WB analysis using 27592-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 27592-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 27592-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 27592-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 27592-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 27592-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 27592-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, L02 cells, HepG2 cells, rat liver tissue
Positive IHC detected in	human liver cancer tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 12 publications below
IF	See 1 publications below
IP	See 3 publications below
CoIP	See 2 publications below
RIP	See 1 publications below

Product Information

27592-1-AP targets RACK1; GNB2L1 in WB, IP, IF, RIP, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Cited Reactivity	human, mouse, monkey, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RACK1; GNB2L1 fusion protein Ag26708 相同性解析による交差性が予測される生物種
Full Name	guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
Calculated molecular weight	36 kDa
Observed molecular weight	31 kDa
GenBank accession number	BC019093
Gene symbol	RACK1
Gene ID (NCBI)	10399
RRID	AB_2880917
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).

Protocols

Product Specific Protocols
WB protocol for RACK1; GNB2L1 antibody 27592-1-AP	Download protocol
IHC protocol for RACK1; GNB2L1 antibody 27592-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,CoIP	J Clin Invest CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages Authors - Guohao Zhang View Article
human	WB,IF,IP	J Virol The matrix protein of respiratory syncytial virus suppresses interferon signaling via RACK1 association Authors - Jingjing Cao View Article
	WB	Cancers (Basel) RACK1 Promotes Meningioma Progression by Activation of NF-κB Pathway via Preventing CSNK2B from Ubiquitination Degradation Authors - Ali Abdi Maalim View Article
monkey	WB	Virology RACK1 promotes porcine reproductive and respiratory syndrome virus infection in Marc-145 cells through ERK1/2 activation Authors - Xinxian Wang KD Validated View Article
human	WB,IP	Ann Transl Med USP41 promotes breast cancer via regulating RACK1. Authors - Meiling Huang View Article
monkey,pig	WB	Vet Microbiol Small molecule screening identified cepharanthine as an inhibitor of porcine reproductive and respiratory syndrome virus infection in vitro by suppressing integrins/ILK/RACK1/PKCα/NF-κB signalling axis. Authors - Chao Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RACK1; GNB2L1 Polyclonal antibody

RACK1; GNB2L1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 27592-1-AP

WB analysis using 27592-1-AP

IHC staining of human liver cancer using 27592-1-AP

IHC staining of human liver cancer using 27592-1-AP

IHC staining of human stomach cancer using 27592-1-AP

IHC staining of human stomach cancer using 27592-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages

The matrix protein of respiratory syncytial virus suppresses interferon signaling via RACK1 association

RACK1 Promotes Meningioma Progression by Activation of NF-κB Pathway via Preventing CSNK2B from Ubiquitination Degradation

RACK1 promotes porcine reproductive and respiratory syndrome virus infection in Marc-145 cells through ERK1/2 activation

USP41 promotes breast cancer via regulating RACK1.

Small molecule screening identified cepharanthine as an inhibitor of porcine reproductive and respiratory syndrome virus infection in vitro by suppressing integrins/ILK/RACK1/PKCα/NF-κB signalling axis.