Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of K-562 using 18333-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 18333-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18333-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 18333-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 18333-1-AP
IP result of anti-RAD18 (IP:18333-1-AP, 3ug; Detection:18333-1-AP 1:300) with K-562 cells lysate 2000ug.
IHC staining of human ovary cancer using 18333-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18333-1-AP (RAD18 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 18333-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18333-1-AP (RAD18 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

RAD18 Polyclonal antibody

RAD18 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 18333-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

RING-type E3 ubiquitin transferase RAD18, RING finger protein 73, hRAD18, hHR18, EC:2.3.2.27

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, A549 cells, HeLa cells, MCF-7 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 2 publications below
IF	See 8 publications below
IP	See 1 publications below

Product Information

18333-1-AP targets RAD18 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAD18 fusion protein Ag12944 相同性解析による交差性が予測される生物種
Full Name	RAD18 homolog (S. cerevisiae)
Calculated molecular weight	56 kDa
Observed molecular weight	75 kDa, 85 kDa
GenBank accession number	BC001302
Gene Symbol	RAD18
Gene ID (NCBI)	56852
RRID	AB_2176586
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NS91
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAD18(E3 ubiquitin-protein ligase RAD18) is lalso named as RNF73, hHR18 and belongs to the RAD18 family. It is involved in postreplication repair of UV-damaged DNA. RAD18 protein is detected in human cells as two major bands at 75 and 85 kDa by Western blot. The bands are identified as nonubiquitinated and monoubiquitinated forms of Rad18, respectively(PMID: 15509568). Monoubiquitinated RAD18 is detected mainly in the cytoplasm, whereas nonubiquitinated RAD18 is detected predominantly in the nuclei(PMID:15509568). This antibody is specific to RAD18.

Protocols

Product Specific Protocols
WB protocol for RAD18 antibody 18333-1-AP	Download protocol
IHC protocol for RAD18 antibody 18333-1-AP	Download protocol
IP protocol for RAD18 antibody 18333-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nat Commun Regulation of the DNA damage response on male meiotic sex chromosomes. Authors - Lu Lin-Yu LY View Article
human	WB	Theranostics A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis. Authors - Qi Zhang View Article
human	IF	Nucleic Acids Res REV1 and polymerase ζ facilitate homologous recombination repair. Authors - Sharma Shilpy S View Article
human	WB	J Cell Sci RNF168 forms a functional complex with RAD6 during the DNA damage response. Authors - Liu Chao C View Article
human	IF	Cell Cycle A small ubiquitin binding domain inhibits ubiquitin-dependent protein recruitment to DNA repair foci. Authors - Corey M Helchowski View Article
human	WB,IF	Mol Pharmacol DNA polymerase ζ is a major determinant of resistance to platinum-based chemotherapeutic agents. Authors - Sharma Shilpy S View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of K-562 using 18333-1-AP

WB analysis of A549 using 18333-1-AP

WB analysis of HeLa using 18333-1-AP

WB analysis of MCF-7 using 18333-1-AP

IP experiment of K-562 using 18333-1-AP

IHC staining of human ovary cancer using 18333-1-AP

IHC staining of human ovary cancer using 18333-1-AP

RAD18 Polyclonal antibody

RAD18 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Regulation of the DNA damage response on male meiotic sex chromosomes.

A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.

REV1 and polymerase ζ facilitate homologous recombination repair.

RNF168 forms a functional complex with RAD6 during the DNA damage response.

A small ubiquitin binding domain inhibits ubiquitin-dependent protein recruitment to DNA repair foci.

DNA polymerase ζ is a major determinant of resistance to platinum-based chemotherapeutic agents.