- Featured Product
- KD/KO Validated
RAD18 Polyclonal antibody
RAD18 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 18333-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, A549 cells, HeLa cells, MCF-7 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 7 publications below |
Product Information
18333-1-AP targets RAD18 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RAD18 fusion protein Ag12944 相同性解析による交差性が予測される生物種 |
| Full Name | RAD18 homolog (S. cerevisiae) |
| Calculated molecular weight | 56 kDa |
| Observed molecular weight | 75 kDa, 85 kDa |
| GenBank accession number | BC001302 |
| Gene symbol | RAD18 |
| Gene ID (NCBI) | 56852 |
| RRID | AB_2176586 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RAD18(E3 ubiquitin-protein ligase RAD18) is lalso named as RNF73, hHR18 and belongs to the RAD18 family. It is involved in postreplication repair of UV-damaged DNA. RAD18 protein is detected in human cells as two major bands at 75 and 85 kDa by Western blot. The bands are identified as nonubiquitinated and monoubiquitinated forms of Rad18, respectively(PMID: 15509568). Monoubiquitinated RAD18 is detected mainly in the cytoplasm, whereas nonubiquitinated RAD18 is detected predominantly in the nuclei(PMID:15509568). This antibody is specific to RAD18.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAD18 antibody 18333-1-AP | Download protocol |
| IHC protocol for RAD18 antibody 18333-1-AP | Download protocol |
| IP protocol for RAD18 antibody 18333-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis. | ||
Cell Cycle A small ubiquitin binding domain inhibits ubiquitin-dependent protein recruitment to DNA repair foci. | ||
Mol Pharmacol DNA polymerase ζ is a major determinant of resistance to platinum-based chemotherapeutic agents. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Alexandra (Verified Customer) (03-20-2024) | The middle part of the picture is irrelevant (other sample, other antibody), but I did not want to modify the picture. The antibody works perfect, on the picture the endogenous Rad18 can be seen. It works fine for the endogenous protein visualisation in 1000x dilution, incubation ON.
 |
