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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD21 Recombinant antibody (84920-4-RR)

    WB analysis using 84920-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84920-4-RR (RAD21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAD21 Recombinant antibody (84920-4-RR)

    WB analysis of HeLa using 84920-4-RR

    WB result of RAD21 antibody (84920-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD21 transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAD21 Recombinant antibody (84920-4-RR)

    IF Staining of HeLa using 84920-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD21 antibody (84920-4-RR, Clone: 242172E3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of Jurkat cells using RAD21 Recombinant antibody (84920-4-RR)

    FC experiment of Jurkat using 84920-4-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RAD21 Recombinant antibody (84920-4-RR, Clone:242172E3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84920-4-RR

    Affinity and Kinetic Characterization of 84920-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84920-4-RR against Human RAD21 were performed. The affinity constant is 2.23 nM.

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  • Featured Product
  • KD/KO Validated

RAD21 Recombinant antibody

RAD21 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84920-4-RR
Clone No.242172E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

hHR21, FLJ40596, FLJ25655, Double-strand-break repair protein rad21 homolog, 64-kDa C-terminal product

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84920-4-RR targets RAD21 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAD21 fusion protein Ag25481 相同性解析による交差性が予測される生物種
Full Name RAD21 homolog (S. pombe)
Calculated molecular weight 631 aa, 72 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC050381
Gene Symbol RAD21
Gene ID (NCBI) 5885
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60216
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAD21, also named as Nuclear matrix protein 1, is a 631 amino acid protein, which belongs to the rad21 family. The calculated molecular weight of RAD21 is 72 kDa, but the phosphorylated RAD21 is about 120 kDa. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

Protocols

Product Specific Protocols
WB protocol for RAD21 antibody 84920-4-RRDownload protocol
IF protocol for RAD21 antibody 84920-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols