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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAD23B Monoclonal antibody, PBS Only

RAD23B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E6G9

Cat no : 67988-1-PBS

Synonyms

HHR23B, HR23B, P58, RAD23B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD23B Monoclonal antibody (67988-1-Ig)

    WB analysis using 67988-1-Ig (same clone as 67988-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67988-1-Ig (RAD23B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RAD23B Monoclonal antibody (67988-1-Ig)

    WB analysis using 67988-1-Ig (same clone as 67988-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67988-1-Ig (RAD23B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RAD23B Monoclonal antibody (67988-1-Ig)

    IHC staining of mouse brain using 67988-1-Ig (same clone as 67988-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67988-1-Ig (RAD23B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RAD23B Monoclonal antibody (67988-1-Ig)

    IHC staining of mouse brain using 67988-1-Ig (same clone as 67988-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67988-1-Ig (RAD23B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAD23B Monoclonal antibody (67988-1-Ig)

    IF Staining of HeLa using 67988-1-Ig (same clone as 67988-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD23B antibody (67988-1-Ig, Clone: 2E6G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67988-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67988-1-PBS targets RAD23B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAD23B fusion protein Ag28658 相同性解析による交差性が予測される生物種
Full Name RAD23 homolog B (S. cerevisiae)
Calculated molecular weight 409 aa, 43 kDa
Observed molecular weight58 kDa
GenBank accession numberBC020973
Gene symbol RAD23B
Gene ID (NCBI) 5887
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B has two subunit: Heterodimer of a 125 kDa subunit (p125) and of a 58 kDa subunit (p58).