Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 29390-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 29390-1-AP (RAD50 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 29390-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 29390-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 29390-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 29390-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 29390-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 29390-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 29390-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RAD50 antibody (29390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

RAD50 Polyclonal antibody

RAD50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 29390-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DNA repair protein RAD50, hRad50, RAD50, RAD50 2, RAD50 homolog (S. cerevisiae)

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	C6 cells, Jurkat cells, K-562 cells, MCF-7 cells, MOLT-4 cells
Positive IHC detected in	human breast cancer tissue, mouse testis tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 1 publications below

Product Information

29390-1-AP targets RAD50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity	Human, rat, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAD50 fusion protein Ag29949 相同性解析による交差性が予測される生物種
Full Name	RAD50 homolog (S. cerevisiae)
Calculated molecular weight	154 kDa
Observed molecular weight	154 kDa
GenBank accession number	NM_005732
Gene Symbol	RAD50
Gene ID (NCBI)	10111
RRID	AB_2918289
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92878
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RAD50 antibody 29390-1-AP	Download protocol
IHC protocol for RAD50 antibody 29390-1-AP	Download protocol
IF protocol for RAD50 antibody 29390-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Transl Med Modulation of SRC by SNTB1 activates the Hippo-YAP pathway during colon adenocarcinoma metastasis Authors - Zhengyan Chang View Article
human	IF	Cell Genom NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors Authors - Yahui Wang View Article
human	WB	CNS Neurosci Ther Deep-targeted gene sequencing reveals ARID1A mutation as an important driver of glioblastoma Authors - Menglin Xiao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 29390-1-AP

IHC staining of human breast cancer using 29390-1-AP

IHC staining of human breast cancer using 29390-1-AP

IHC staining of mouse testis using 29390-1-AP

IHC staining of mouse testis using 29390-1-AP

IHC staining of human placenta using 29390-1-AP