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RAD50 Polyclonal antibody

RAD50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29390-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

DNA repair protein RAD50, hRad50, RAD50, RAD50 2, RAD50 homolog (S. cerevisiae)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inC6 cells, Jurkat cells, K-562 cells, MCF-7 cells, MOLT-4 cells
Positive IHC detected inhuman breast cancer tissue, mouse testis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29390-1-AP targets RAD50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAD50 fusion protein Ag29949 相同性解析による交差性が予測される生物種
Full Name RAD50 homolog (S. cerevisiae)
Calculated molecular weight 154 kDa
Observed molecular weight154 kDa
GenBank accession numberNM_005732
Gene Symbol RAD50
Gene ID (NCBI) 10111
RRIDAB_2918289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92878
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAD50 antibody 29390-1-APDownload protocol
IHC protocol for RAD50 antibody 29390-1-APDownload protocol
IF protocol for RAD50 antibody 29390-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Transl Med

Modulation of SRC by SNTB1 activates the Hippo-YAP pathway during colon adenocarcinoma metastasis

Authors - Zhengyan Chang
humanIF

Cell Genom

NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors

Authors - Yahui Wang
humanWB

CNS Neurosci Ther

Deep-targeted gene sequencing reveals ARID1A mutation as an important driver of glioblastoma

Authors - Menglin Xiao
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