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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAD50 Polyclonal antibody

RAD50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29390-1-AP

Synonyms

DNA repair protein RAD50, hRad50, RAD50, RAD50 2, RAD50 homolog (S. cerevisiae)

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD50 Polyclonal antibody (29390-1-AP)

    WB analysis using 29390-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29390-1-AP (RAD50 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAD50 Polyclonal antibody (29390-1-AP)

    IHC staining of human breast cancer using 29390-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAD50 Polyclonal antibody (29390-1-AP)

    IHC staining of human breast cancer using 29390-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RAD50 Polyclonal antibody (29390-1-AP)

    IHC staining of mouse testis using 29390-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RAD50 Polyclonal antibody (29390-1-AP)

    IHC staining of mouse testis using 29390-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RAD50 Polyclonal antibody (29390-1-AP)

    IHC staining of human placenta using 29390-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RAD50 Polyclonal antibody (29390-1-AP)

    IHC staining of human placenta using 29390-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RAD50 Polyclonal antibody (29390-1-AP)

    IF Staining of U2OS using 29390-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RAD50 antibody (29390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inC6 cells, Jurkat cells, K-562 cells, MCF-7 cells, MOLT-4 cells
Positive IHC detected inhuman breast cancer tissue, mouse testis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29390-1-AP targets RAD50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAD50 fusion protein Ag29949 相同性解析による交差性が予測される生物種
Full Name RAD50 homolog (S. cerevisiae)
Calculated molecular weight 154 kDa
Observed molecular weight154 kDa
GenBank accession numberNM_005732
Gene symbol RAD50
Gene ID (NCBI) 10111
RRIDAB_2918289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAD50 antibody 29390-1-APDownload protocol
IHC protocol for RAD50 antibody 29390-1-APDownload protocol
IF protocol for RAD50 antibody 29390-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Genom

NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors

Authors - Yahui Wang
View Article
humanWB

CNS Neurosci Ther

Deep-targeted gene sequencing reveals ARID1A mutation as an important driver of glioblastoma

Authors - Menglin Xiao
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Veronica (Verified Customer) (11-14-2022)

The antibody seems to work correctly. However, further tests need to be done.

  • Applications: Immunofluorescence
  • Cell Tissue Type: hTERT RPE-1