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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD51AP1 Monoclonal antibody (68560-1-Ig)

    WB analysis using 68560-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68560-1-Ig (RAD51AP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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RAD51AP1 Monoclonal antibody

RAD51AP1 Monoclonal Antibody for WB, ELISA
Cat No. 68560-1-Ig
Clone No.2D8B1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

RAD51-interacting protein, RAD51-associated protein 1, RAD51 interacting protein, RAD51 associated protein 1, PIR51

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, SW480 cells, NCCIT cells, Daudi cells, HaCaT cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68560-1-Ig targets RAD51AP1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAD51AP1 fusion protein Ag28385 相同性解析による交差性が予測される生物種
Full Name RAD51 associated protein 1
Calculated molecular weight 38 kDa
Observed molecular weight38-40 kDa
GenBank accession numberBC016330
Gene Symbol RAD51AP1
Gene ID (NCBI) 10635
RRIDAB_3085262
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96B01
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].

Protocols

Product Specific Protocols
WB protocol for RAD51AP1 antibody 68560-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols