Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293T cells, HeLa cells, MDA-MB-231 cells, MCF-7 cells, T-47D cells, mouse testis tissue |
Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
84537-5-RR targets RAD51C in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | RAD51C fusion protein Ag35070 相同性解析による交差性が予測される生物種 |
Full Name | RAD51 homolog C (S. cerevisiae) |
Calculated molecular weight | 376 aa, 42 kDa |
Observed molecular weight | 40-42 kDa |
GenBank accession number | BC107753 |
Gene Symbol | RAD51C |
Gene ID (NCBI) | 5889 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | O43502 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RAD51C (DNA repair protein RAD51 homolog 3) is essential for the homologous recombination (HR) pathway of DNA repair and is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents (PMID: 14716019; 16215984; 16395335).
Protocols
Product Specific Protocols | |
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WB protocol for RAD51C antibody 84537-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |