• Featured Product
  • KD/KO Validated

RAD52 Polyclonal antibody

RAD52 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 28045-1-AP

Synonyms

RAD52, RAD52 homolog (S. cerevisiae)


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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HL-60 cells, HeLa cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28045-1-AP targets RAD52 in WB, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAD52 fusion protein Ag27619 相同性解析による交差性が予測される生物種
Full Name RAD52 homolog (S. cerevisiae)
Calculated molecular weight 46 aa
Observed molecular weight 46-50 kDa
GenBank accession numberNM_001297419
Gene symbol RAD52
Gene ID (NCBI) 5893
RRIDAB_2881046
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAD52 antibody 28045-1-APDownload protocol
IP protocol for RAD52 antibody 28045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Mol Cell

EXO1 protects BRCA1-deficient cells against toxic DNA lesions

Authors - Bert van de Kooij
humanWB

Sci Adv

Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair

Authors - Dinh Duc Nguyen
humanWB

J Cell Mol Med

RDM1 promotes critical processes in breast cancer tumorigenesis.

Authors - Yajun Chen
pigWB

Int J Biochem Cell Biol

Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.

Authors - Guoling Li
humanWB

Cell Death Dis

SF3B4 promotes ovarian cancer progression by regulating alternative splicing of RAD52.

Authors - Yuchao Diao
  • KD Validated
humanWB

Nat Commun

Pathway choice in the alternative telomere lengthening in neoplasia is dictated by replication fork processing mediated by EXD2's nuclease activity

Authors - Ronan Broderick
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Daniel (Verified Customer) (10-15-2024)

I detected the expected band for RAD52 and 48 kDa in WT cells, but not RAD52 KO cells, proving that the antibody is indeed recognizing RAD52.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HCT116 and RPE1 cells