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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using RAD52 Polyclonal antibody (55020-1-AP)

    WB analysis of PC-3 using 55020-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 55020-1-AP (RAD52 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using RAD52 Polyclonal antibody (55020-1-AP)

    IP experiment of PC-3 using 55020-1-AP

    IP result of anti-RAD52 (IP:55020-1-AP, 4ug; Detection:55020-1-AP 1:300) with PC-3 cells lysate 3200ug.

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RAD52 Polyclonal antibody

RAD52 Polyclonal Antibody for WB, IP, ELISA
Cat No. 55020-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

RAD52, RAD52 homolog (S. cerevisiae)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells
Positive IP detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55020-1-AP targets RAD52 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name RAD52 homolog (S. cerevisiae)
Calculated molecular weight 46 kDa
GenBank accession numberNM_134424
Gene Symbol RAD52
Gene ID (NCBI) 5893
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43351
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.