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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD52 Monoclonal antibody (68283-1-Ig)

    WB analysis using 68283-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68283-1-Ig (RAD52 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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RAD52 Monoclonal antibody

RAD52 Monoclonal Antibody for WB, ELISA
Cat No. 68283-1-Ig
Clone No.3H8G9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

DNA repair protein RAD52 homolog, 3H8G9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HL-60 cells, HSC-T6 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68283-1-Ig targets RAD52 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAD52 fusion protein Ag27665 相同性解析による交差性が予測される生物種
Full Name RAD52 homolog (S. cerevisiae)
Calculated molecular weight 46 aa
Observed molecular weight46 kDa
GenBank accession numberNM_001297419
Gene Symbol RAD52
Gene ID (NCBI) 5893
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43351
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In Saccharomyces cerevisiae, Rad52 functions as a critical mediator of homologous recombination (HR) by facilitating the loading of Rad51 onto ssDNA. However, in mammalian systems and other vertebrates, BRCA2 is known to function as the primary mediator of RAD51 filament formation, while prior studies have shown a limited role for RAD52 in HR.Additionally, loss of RAD52 has little consequence for cell viability. Recent evidence suggests that RAD52 may provide an alternative mediator pathway to BRCA2 function (PMID: 34928169). Several studies have suggested a link between the functional regulation of RAD52 and the protein modification by a small ubiquitin-like modifier (SUMO), sumoylation may play an important role in the nuclear transport of RAD52 (PMID:20190268). The calculated MW of Rad52 is 46 kDa, 68283-1-Ig can detect the band around 46 kDa.

Protocols

Product Specific Protocols
WB protocol for RAD52 antibody 68283-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols