Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
68283-1-PBS targets RAD52 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RAD52 fusion protein Ag27665 相同性解析による交差性が予測される生物種 |
| Full Name | RAD52 homolog (S. cerevisiae) |
| Calculated molecular weight | 46 aa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | NM_001297419 |
| Gene Symbol | RAD52 |
| Gene ID (NCBI) | 5893 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P43351 |
| Storage Buffer | PBS only , pH 7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
In Saccharomyces cerevisiae, Rad52 functions as a critical mediator of homologous recombination (HR) by facilitating the loading of Rad51 onto ssDNA. However, in mammalian systems and other vertebrates, BRCA2 is known to function as the primary mediator of RAD51 filament formation, while prior studies have shown a limited role for RAD52 in HR.Additionally, loss of RAD52 has little consequence for cell viability. Recent evidence suggests that RAD52 may provide an alternative mediator pathway to BRCA2 function (PMID: 34928169). Several studies have suggested a link between the functional regulation of RAD52 and the protein modification by a small ubiquitin-like modifier (SUMO), sumoylation may play an important role in the nuclear transport of RAD52 (PMID:20190268). The calculated MW of Rad52 is 46 kDa, 68283-1-Ig can detect the band around 46 kDa.