RAD9A Polyclonal antibody

Name: RAD9A Polyclonal antibody
Brand: Proteintech
SKU: 13035-1-AP

RAD9A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13035-1-AP

Synonyms

RAD9, hRAD9, EC:3.1.11.2, Cell cycle checkpoint control protein RAD9A

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of HeLa using 13035-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 13035-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 13035-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 13035-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13035-1-AP (RAD9A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13035-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13035-1-AP (RAD9A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 13035-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13035-1-AP (RAD9A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 13035-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, MCF-7 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

13035-1-AP targets RAD9A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAD9A fusion protein Ag3687 相同性解析による交差性が予測される生物種
Full Name	RAD9 homolog A (S. pombe)
Calculated molecular weight	391 aa, 42 kDa
Observed molecular weight	55-60 kDa
GenBank accession number	BC014848
Gene symbol	RAD9A
Gene ID (NCBI)	5883
RRID	AB_10598474
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RAD9A antibody 13035-1-AP	Download protocol
IHC protocol for RAD9A antibody 13035-1-AP	Download protocol
IF protocol for RAD9A antibody 13035-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	EMBO J YTHDC1 delays cellular senescence and pulmonary fibrosis by activating ATR in an m6A-independent manner Authors - Canfeng Zhang View Article
human	WB	Oncogene DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity. Authors - Bin Chen View Article
human	WB	J Mol Cell Biol SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress. Authors - Xiaoli Xu View Article
human	WB	DNA Cell Biol AEG-1 Knockdown Sensitizes Glioma Cells to Radiation Through Impairing Homologous Recombination Via Targeting RFC5. Authors - Xu Zhao View Article

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企業情報

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAD9A Polyclonal antibody

RAD9A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HeLa using 13035-1-AP

WB analysis of A549 using 13035-1-AP

WB analysis of MCF-7 using 13035-1-AP

IHC staining of human breast cancer using 13035-1-AP

IHC staining of human breast cancer using 13035-1-AP

IF Staining of HeLa using 13035-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

YTHDC1 delays cellular senescence and pulmonary fibrosis by activating ATR in an m6A-independent manner

DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.

SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.

AEG-1 Knockdown Sensitizes Glioma Cells to Radiation Through Impairing Homologous Recombination Via Targeting RFC5.