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RADIL Polyclonal antibody
RADIL Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 20284-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, SH-SY5Y cells, mouse testis tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
20284-1-AP targets RADIL in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RADIL fusion protein Ag13996 相同性解析による交差性が予測される生物種 |
| Full Name | Ras association and DIL domains |
| Calculated molecular weight | 1075 aa, 117 kDa |
| Observed molecular weight | 117 kDa |
| GenBank accession number | BC004919 |
| Gene symbol | RADIL |
| Gene ID (NCBI) | 55698 |
| RRID | AB_10732684 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Radil is a broadly expressed adapter protein that contains a RA-like domain, a PDZ domain and a DIL domain. Like RAPL and RIAM, Radil interacts preferentially with active GTP-bound Rap1, and overexpression of Radil enhances integrin-mediated adhesion. In addition, Radil knockdown inhibits cell-substrate adhesion in HT1080, AD293, and NMuMG cells and results in migration defects in SW1353 and zebrafish neural crest cells (PMID: 17704304). Radil regulates neutrophil adhesion by specifically enhancing β2-integrin activation, as well as focal adhesion kinase (FAK) and paxillin phosphorylation (PMID: 23097489).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RADIL antibody 20284-1-AP | Download protocol |
| IF protocol for RADIL antibody 20284-1-AP | Download protocol |
| IP protocol for RADIL antibody 20284-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancers (Basel) Proteomic Mapping of the Interactome of KRAS Mutants Identifies New Features of RAS Signalling Networks and the Mechanism of Action of Sotorasib | ||
J Biol Chem Identification of Radil as a Ras binding partner and putative activator.
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