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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAE1 Recombinant antibody (83712-4-RR)

    WB analysis using 83712-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83712-4-RR (RAE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RAE1 Recombinant antibody (83712-4-RR)

    IHC staining of mouse testis using 83712-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83712-4-RR (RAE1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RAE1 Recombinant antibody (83712-4-RR)

    IF Staining of HepG2 using 83712-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RAE1 antibody (83712-4-RR, Clone: 240861B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83712-4-RR

    Affinity and Kinetic Characterization of 83712-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83712-4-RR against Human RAE1 were performed. The affinity constant is 20.7 nM.

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RAE1 Recombinant antibody

RAE1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83712-4-RR
Clone No.240861B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, HeLa cells, HEK-293 cells, BxPC-3 cells, mouse thymus tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83712-4-RR targets RAE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAE1 fusion protein Ag12102 相同性解析による交差性が予測される生物種
Full Name RAE1 RNA export 1 homolog (S. pombe)
Calculated molecular weight 368 aa, 41 kDa
GenBank accession numberBC103754
Gene Symbol RAE1
Gene ID (NCBI) 8480
RRIDAB_3671316
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP78406
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)

Protocols

Product Specific Protocols
WB protocol for RAE1 antibody 83712-4-RRDownload protocol
IHC protocol for RAE1 antibody 83712-4-RRDownload protocol
IF protocol for RAE1 antibody 83712-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols