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  • Featured Product
  • KD/KO Validated

RAF1 Monoclonal antibody, PBS Only

RAF1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D6A1

Cat no : 66592-1-PBS

Synonyms

c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAF1 Monoclonal antibody (66592-1-Ig)

    WB analysis using 66592-1-Ig (same clone as 66592-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66592-1-Ig (RAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RAF1 Monoclonal antibody (66592-1-Ig)

    WB analysis of HEK-293 using 66592-1-Ig (same clone as 66592-1-PBS)

    WB result of RAF1 antibody (66592-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAF1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DC2.4 cells using RAF1 Monoclonal antibody (66592-1-Ig)

    WB analysis of DC2.4 using 66592-1-Ig (same clone as 66592-1-PBS)

    DC2.4 cells were subjected to SDS PAGE followed by western blot with 66592-1-Ig (RAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RAF1 Monoclonal antibody (66592-1-Ig)

    IHC staining of human prostate cancer using 66592-1-Ig (same clone as 66592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66592-1-Ig (RAF1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RAF1 Monoclonal antibody (66592-1-Ig)

    IHC staining of human prostate cancer using 66592-1-Ig (same clone as 66592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66592-1-Ig (RAF1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RAF1 Monoclonal antibody (66592-1-Ig)

    IF Staining of HEK-293 using 66592-1-Ig (same clone as 66592-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RAF1 antibody (66592-1-Ig, Clone: 1D6A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (Blue). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66592-1-PBS targets RAF1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAF1 fusion protein Ag25423 相同性解析による交差性が予測される生物種
Full Name v-raf-1 murine leukemia viral oncogene homolog 1
Calculated molecular weight 648 aa, 73 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC018119
Gene symbol RAF1
Gene ID (NCBI) 5894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.