RAF1 Monoclonal antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 66592-1-Ig (RAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

WB result of RAF1 antibody (66592-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAF1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

DC2.4 cells were subjected to SDS PAGE followed by western blot with 66592-1-Ig (RAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66592-1-Ig (RAF1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66592-1-Ig (RAF1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RAF1 antibody (66592-1-Ig, Clone: 1D6A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.