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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAG2 Monoclonal antibody, PBS Only

RAG2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B4E5

Cat no : 66998-1-PBS

Synonyms

V(D)J recombination-activating protein 2, RAG-2, RAG 2, 1B4E5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAG2 Monoclonal antibody (66998-1-Ig)

    WB analysis using 66998-1-Ig (same clone as 66998-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66998-1-Ig (RAG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66998-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RAG2 Monoclonal antibody (66998-1-Ig)

    IF Staining of HepG2 using 66998-1-Ig (same clone as 66998-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RAG2 antibody (66998-1-Ig, Clone: 1B4E5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66998-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using RAG2 Monoclonal antibody (66998-1-Ig)

    FC experiment of HepG2 using 66998-1-Ig (same clone as 66998-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human RAG2 (66998-1-Ig, Clone:1B4E5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66998-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66998-1-PBS targets RAG2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAG2 fusion protein Ag16780 相同性解析による交差性が予測される生物種
Full Name recombination activating gene 2
Calculated molecular weight 527 aa, 59 kDa
Observed molecular weight 57-62 kDa
GenBank accession numberBC022397
Gene symbol RAG2
Gene ID (NCBI) 5897
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3