Validation Data Gallery

Filter: AllWBIHC

WB analysis of Y79 using 12340-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12340-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 12340-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 12340-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 12340-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12340-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 12340-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12340-1-AP (RALB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 12340-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12340-1-AP (RALB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 12340-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12340-1-AP (RALB antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human colon using 12340-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12340-1-AP (RALB antibody) at dilution of 1:200 (under 40x lens).

RALB Polyclonal antibody

RALB Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12340-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

RALB, Ras related protein Ral B

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Y79 cells, human brain tissue, human lung tissue, mouse brain tissue, mouse lung tissue
Positive IHC detected in	human ovary tumor tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 2 publications below

Product Information

12340-1-AP targets RALB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	Drosophila, human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RALB fusion protein Ag2992 相同性解析による交差性が予測される生物種
Full Name	v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
Calculated molecular weight	206 aa, 23 kDa
Observed molecular weight	23 kDa
GenBank accession number	BC018163
Gene Symbol	RALB
Gene ID (NCBI)	5899
RRID	AB_2269253
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P11234
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RALB antibody 12340-1-AP	Download protocol
IHC protocol for RALB antibody 12340-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
Drosophila	IHC	Development Ral GTPase promotes asymmetric Notch activation in the Drosophila eye in response to Frizzled/PCP signaling by repressing ligand-independent receptor activation. Authors - Cho Bomsoo B View Article
human	WB	Front Chem Design, Synthesis, and Biological Evaluation of Pyrano[2,3-c]-pyrazole-Based RalA Inhibitors Against Hepatocellular Carcinoma. Authors - Yuting Wang View Article
human	WB,IHC	Arch Biochem Biophys MicroRNA-139 inhibits pancreatic-cancer carcinogenesis by suppressing RalB via the Ral/RAC/PI3K pathway. Authors - Yan Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Y79 using 12340-1-AP

WB analysis of human brain using 12340-1-AP

WB analysis of human lung using 12340-1-AP

WB analysis of Y79 using 12340-1-AP

WB analysis of mouse lung using 12340-1-AP

WB analysis of mouse brain using 12340-1-AP

IHC staining of human ovary tumor using 12340-1-AP