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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RALBP1 Polyclonal antibody

RALBP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13176-1-AP

Synonyms

EC:7.6.2.2, EC:7.6.2.3, DNP-SG ATPase, DNP SG ATPase, Dinitrophenyl S-glutathione ATPase, 76 kDa Ral-interacting protein

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using RALBP1 Polyclonal antibody (13176-1-AP)

    WB analysis of K-562 using 13176-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13176-1-AP (RALBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RALBP1 Polyclonal antibody (13176-1-AP)

    WB analysis of HEK-293 using 13176-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13176-1-AP (RALBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RALBP1 Polyclonal antibody (13176-1-AP)

    WB analysis of HepG2 using 13176-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13176-1-AP (RALBP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RALBP1 Polyclonal antibody (13176-1-AP)

    WB analysis of MCF-7 using 13176-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13176-1-AP (RALBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using RALBP1 Polyclonal antibody (13176-1-AP)

    IP experiment of K-562 using 13176-1-AP

    IP result of anti-RALBP1 (IP:13176-1-AP, 4ug; Detection:13176-1-AP 1:800) with K-562 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RALBP1 Polyclonal antibody (13176-1-AP)

    IHC staining of human stomach using 13176-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13176-1-AP (RALBP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, HepG2 cells, MCF-7 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13176-1-AP targets RALBP1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RALBP1 fusion protein Ag3843 相同性解析による交差性が予測される生物種
Full Name ralA binding protein 1
Calculated molecular weight 655 aa, 76 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC013126
Gene symbol RALBP1
Gene ID (NCBI) 10928
RRIDAB_2176171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RALBP1 antibody 13176-1-APDownload protocol
IHC protocol for RALBP1 antibody 13176-1-APDownload protocol
IP protocol for RALBP1 antibody 13176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Neoplasia

PMID: 28092822

Authors - Jian Yang
View Article