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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using RALY Monoclonal antibody (68011-1-Ig)

    WB analysis of LNCaP using 68011-1-Ig (same clone as 68011-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68011-1-Ig (RALY antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RALY Monoclonal antibody (68011-1-Ig)

    IHC staining of human stomach cancer using 68011-1-Ig (same clone as 68011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68011-1-Ig (RALY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RALY Monoclonal antibody (68011-1-Ig)

    IHC staining of human stomach cancer using 68011-1-Ig (same clone as 68011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68011-1-Ig (RALY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RALY Monoclonal antibody (68011-1-Ig)

    IF Staining of A431 using 68011-1-Ig (same clone as 68011-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RALY antibody (68011-1-Ig, Clone: 2B10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using RALY Monoclonal antibody (68011-1-Ig)

    FC experiment of A431 using 68011-1-Ig (same clone as 68011-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RALY (68011-1-Ig, Clone:2B10C3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

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RALY Monoclonal antibody, PBS Only

RALY Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68011-1-PBS
Clone No.2B10C3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

HNRPCL2, hnRNP core protein C-like 2, hnRNP associated with lethal yellow protein homolog, Heterogeneous nuclear ribonucleoprotein C-like 2, Autoantigen p542

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68011-1-PBS targets RALY in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RALY fusion protein Ag12250 相同性解析による交差性が予測される生物種
Full Name RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse))
Calculated molecular weight 307 aa, 33 kDa
Observed molecular weight30-37 kDa
GenBank accession numberBC103753
Gene Symbol RALY
Gene ID (NCBI) 22913
RRIDAB_2918758
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UKM9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RNA-binding protein RALY is a protein that in humans is encoded by the RALY gene. In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family.