RALY Monoclonal antibody, PBS Only

LNCaP cells were subjected to SDS PAGE followed by western blot with 68011-1-Ig (RALY antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68011-1-Ig (RALY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68011-1-Ig (RALY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed A431 cells using RALY antibody (68011-1-Ig, Clone: 2B10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.

1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RALY (68011-1-Ig, Clone:2B10C3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68011-1-PBS in a different storage buffer formulation.