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Species Reactivity

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  • Featured Product
  • KD/KO Validated

RAN Polyclonal antibody

RAN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10469-1-AP

Synonyms

ARA24, Androgen receptor-associated protein 24, GTP-binding nuclear protein Ran, GTPase Ran, GTP binding nuclear protein Ran

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RAN Polyclonal antibody (10469-1-AP)

    WB analysis of HEK-293 using 10469-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using RAN Polyclonal antibody (10469-1-AP)

    WB analysis of A375 using 10469-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using RAN Polyclonal antibody (10469-1-AP)

    WB analysis of mouse kidney using 10469-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using RAN Polyclonal antibody (10469-1-AP)

    WB analysis of A375 using 10469-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using RAN Polyclonal antibody (10469-1-AP)

    WB analysis of rat kidney using 10469-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RAN Polyclonal antibody (10469-1-AP)

    WB analysis of NIH/3T3 using 10469-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAN Polyclonal antibody (10469-1-AP)

    WB analysis of HeLa using 10469-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RAN Polyclonal antibody (10469-1-AP)

    WB analysis of Jurkat using 10469-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using RAN Polyclonal antibody (10469-1-AP)

    IP experiment of mouse kidney using 10469-1-AP

    IP result of anti-RAN (IP:10469-1-AP, 4ug; Detection:10469-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAN Polyclonal antibody (10469-1-AP)

    IHC staining of human breast cancer using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAN Polyclonal antibody (10469-1-AP)

    IHC staining of human breast cancer using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using RAN Polyclonal antibody (10469-1-AP)

    IHC staining of mouse colon using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RAN Polyclonal antibody (10469-1-AP)

    IHC staining of mouse testis using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using RAN Polyclonal antibody (10469-1-AP)

    IHC staining of rat colon using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RAN Polyclonal antibody (10469-1-AP)

    IHC staining of human placenta using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using RAN Polyclonal antibody (10469-1-AP)

    IHC staining of human colon using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded normal colon slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using RAN Polyclonal antibody (10469-1-AP)

    IHC staining of human testis using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 10469-1-AP (RAN antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RAN Polyclonal antibody (10469-1-AP)

    IHC staining of human testis using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 10469-1-AP (RAN antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RAN Polyclonal antibody (10469-1-AP)

    IHC staining of human testis using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10469-1-AP (RAN Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RAN Polyclonal antibody (10469-1-AP)

    IHC staining of human testis using 10469-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10469-1-AP (RAN Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RAN Polyclonal antibody (10469-1-AP)

    IF Staining of HEK-293 using 10469-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RAN antibody (10469-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, A375 cells, rat kidney tissue, NIH/3T3 cells, mouse kidney tissue, HeLa cells, Jurkat cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman breast cancer tissue, human colon tissue, human placenta tissue, human testis tissue, mouse colon tissue, mouse testis tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10469-1-AP targets RAN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, macrobrachium rosenbergii
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAN fusion protein Ag0748 相同性解析による交差性が予測される生物種
Full Name RAN, member RAS oncogene family
Calculated molecular weight 25 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC004272
Gene symbol RAN
Gene ID (NCBI) 5901
RRIDAB_2176484
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAN antibody 10469-1-APDownload protocol
IHC protocol for RAN antibody 10469-1-APDownload protocol
IF protocol for RAN antibody 10469-1-APDownload protocol
IP protocol for RAN antibody 10469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IP

Cell Death Dis

RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression.

Authors - Xunhua Liu
  • KD Validated
View Article
human,ratWB

Diabetologia

Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.

Authors - Mu Zhang
View Article
ratIP,WB

Cell Commun Signal

Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.

Authors - Abiola Abdulrahman Ayanlaja
View Article
humanIHC

Oncotarget

Identification of nasopharyngeal carcinoma metastasis-related biomarkers by iTRAQ combined with 2D-LC-MS/MS.

Authors - Zhen Chen
View Article
humanWB

Int J Biol Sci

Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression.

Authors - Yancheng Zhong
  • KD Validated
View Article
humanIF

J Cell Sci

A nuclear export sequence promotes CRM1-dependent targeting of the nucleoporin Nup214 to the nuclear pore complex.

Authors - Mohamed Hamed
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

María (Verified Customer) (02-08-2022)

Works very well for WB (1:1000, 1h) and IF in permeabilized cells (1:200). Validated with siRNA

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: RPE-1/MSC