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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAN Monoclonal antibody, PBS Only

RAN Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B11E1

Cat no : 67500-1-PBS

Synonyms

ARA24, Gsp1, GTPase Ran, RAN, Ras like protein TC4, Ras related nuclear protein, TC4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAN Monoclonal antibody (67500-1-Ig)

    WB analysis using 67500-1-Ig (same clone as 67500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67500-1-Ig (RAN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAN Monoclonal antibody (67500-1-Ig)

    IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAN Monoclonal antibody (67500-1-Ig)

    IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAN Monoclonal antibody (67500-1-Ig)

    IHC staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAN Monoclonal antibody (67500-1-Ig)

    IHC staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using RAN Monoclonal antibody (67500-1-Ig)

    IF Staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using RAN antibody (67500-1-Ig, Clone: 1B11E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67500-1-PBS targets RAN in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RAN fusion protein Ag30034 相同性解析による交差性が予測される生物種
Full Name RAN, member RAS oncogene family
Calculated molecular weight 25 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC004272
Gene symbol RAN
Gene ID (NCBI) 5901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).