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RANBP9 Polyclonal antibody

RANBP9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17755-1-AP

Synonyms

BPM L, BPM90, RAN binding protein 9, Ran binding protein M, RanBP7, RANBP9, RANBPM


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Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, mouse testis
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human skin tissue, mouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17755-1-AP targets RANBP9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RANBP9 fusion protein Ag12112 相同性解析による交差性が予測される生物種
Full Name RAN binding protein 9
Calculated molecular weight388aa,43 kDa; 729aa,78 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC063849
Gene symbol RANBP9
Gene ID (NCBI) 10048
RRIDAB_2176803
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RANBP9 antibody 17755-1-APDownload protocol
IHC protocol for RANBP9 antibody 17755-1-APDownload protocol
IF protocol for RANBP9 antibody 17755-1-APDownload protocol
IP protocol for RANBP9 antibody 17755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

mSphere

B Cell Receptor-Responsive miR-141 Enhances Epstein-Barr Virus Lytic Cycle via FOXO3 Inhibition.

Authors - Yan Chen
humanWB

Oncol Lett

RANBP9 suppresses tumor proliferation in colorectal cancer.

Authors - Chunzhi Qin
ratIF

Acta Histochem

MicroRNA-26a-5p alleviates neuronal apoptosis and brain injury in intracerebral hemorrhage by targeting RAN binding protein 9.

Authors - Huiyuan Zhang
humanIHC

Transl Cancer Res

Reduced RANBP9 expression is associated with poor prognosis in colorectal cancer patients.

Authors - Guangbin Wu