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RANBP9 Polyclonal antibody
RANBP9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, Canine
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 17755-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, HeLa cells, mouse testis |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human testis tissue, human skin tissue, mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MDCK cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
17755-1-AP targets RANBP9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
Tested Reactivity | human, mouse, rat, Canine |
Cited Reactivity | human, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RANBP9 fusion protein Ag12112 相同性解析による交差性が予測される生物種 |
Full Name | RAN binding protein 9 |
Calculated molecular weight | 388aa,43 kDa; 729aa,78 kDa |
Observed molecular weight | 80-90 kDa |
GenBank accession number | BC063849 |
Gene symbol | RANBP9 |
Gene ID (NCBI) | 10048 |
RRID | AB_2176803 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for RANBP9 antibody 17755-1-AP | Download protocol |
IHC protocol for RANBP9 antibody 17755-1-AP | Download protocol |
IF protocol for RANBP9 antibody 17755-1-AP | Download protocol |
IP protocol for RANBP9 antibody 17755-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
mSphere B Cell Receptor-Responsive miR-141 Enhances Epstein-Barr Virus Lytic Cycle via FOXO3 Inhibition. | ||
Acta Histochem MicroRNA-26a-5p alleviates neuronal apoptosis and brain injury in intracerebral hemorrhage by targeting RAN binding protein 9. | ||
Transl Cancer Res Reduced RANBP9 expression is associated with poor prognosis in colorectal cancer patients. |