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RANBP9 Polyclonal antibody

RANBP9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17755-1-AP

Synonyms

BPM L, BPM90, RAN binding protein 9, Ran binding protein M, RanBP7, RANBP9, RANBPM

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RANBP9 Polyclonal antibody (17755-1-AP)

    WB analysis of HeLa using 17755-1-AP

    WB result of RANBP9 antibody (17755-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RANBP9 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using RANBP9 Polyclonal antibody (17755-1-AP)

    WB analysis using 17755-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17755-1-AP (RANBP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RANBP9 Polyclonal antibody (17755-1-AP)

    IP experiment of HeLa using 17755-1-AP

    IP result of anti-RANBP9 (IP:17755-1-AP, 4ug; Detection:17755-1-AP 1:600) with HeLa cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using RANBP9 Polyclonal antibody (17755-1-AP)

    IHC staining of human testis using 17755-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RANBP9 Polyclonal antibody (17755-1-AP)

    IHC staining of human testis using 17755-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using RANBP9 Polyclonal antibody (17755-1-AP)

    IHC staining of human skin using 17755-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat testis tissue using RANBP9 Polyclonal antibody (17755-1-AP)

    IHC staining of rat testis using 17755-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat testis tissue using RANBP9 Polyclonal antibody (17755-1-AP)

    IHC staining of rat testis using 17755-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RANBP9 Polyclonal antibody (17755-1-AP)

    IHC staining of mouse testis using 17755-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RANBP9 Polyclonal antibody (17755-1-AP)

    IHC staining of mouse testis using 17755-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using RANBP9 Polyclonal antibody (17755-1-AP)

    IF Staining of MDCK using 17755-1-AP

    Immunofluorescent analysis of MDCK cells using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RANBP9 Polyclonal antibody (17755-1-AP)

    IF Staining of HeLa using 17755-1-AP

    Immunofluorescent analysis of HeLa cells using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, mouse testis
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human skin tissue, mouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17755-1-AP targets RANBP9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RANBP9 fusion protein Ag12112 相同性解析による交差性が予測される生物種
Full Name RAN binding protein 9
Calculated molecular weight388aa,43 kDa; 729aa,78 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC063849
Gene symbol RANBP9
Gene ID (NCBI) 10048
RRIDAB_2176803
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RANBP9 antibody 17755-1-APDownload protocol
IHC protocol for RANBP9 antibody 17755-1-APDownload protocol
IF protocol for RANBP9 antibody 17755-1-APDownload protocol
IP protocol for RANBP9 antibody 17755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

mSphere

B Cell Receptor-Responsive miR-141 Enhances Epstein-Barr Virus Lytic Cycle via FOXO3 Inhibition.

Authors - Yan Chen
View Article
humanWB

Oncol Lett

RANBP9 suppresses tumor proliferation in colorectal cancer.

Authors - Chunzhi Qin
View Article
ratIF

Acta Histochem

MicroRNA-26a-5p alleviates neuronal apoptosis and brain injury in intracerebral hemorrhage by targeting RAN binding protein 9.

Authors - Huiyuan Zhang
View Article
humanIHC

Transl Cancer Res

Reduced RANBP9 expression is associated with poor prognosis in colorectal cancer patients.

Authors - Guangbin Wu
View Article