Featured Product
KD/KO Validated

RANGAP1 Polyclonal antibody

Name: RANGAP1 Polyclonal antibody
Brand: Proteintech
SKU: 11102-1-AP

RANGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11102-1-AP

Synonyms

SD, Ran GTPase-activating protein 1, KIAA1835

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 11102-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11102-1-AP (RANGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11102-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11102-1-AP (RANGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 11102-1-AP
IP result of anti-RANGAP1 (IP:11102-1-AP, 4ug; Detection:11102-1-AP 1:300) with HEK-293 cells lysate 3600ug.
IHC staining of human gliomas using 11102-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11102-1-AP (RANGAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 11102-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11102-1-AP (RANGAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 11102-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11102-1-AP (RANGAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

-/ -

Cat No. 11102-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	SH-SY5Y cells, HEK-293 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 2 publications below
IF	See 1 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

11102-1-AP targets RANGAP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RANGAP1 fusion protein Ag1563 相同性解析による交差性が予測される生物種
Full Name	Ran GTPase activating protein 1
Calculated molecular weight	64 kDa
Observed molecular weight	64 kDa
GenBank accession number	BC014044
Gene symbol	RANGAP1
Gene ID (NCBI)	5905
RRID	AB_2176968
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RANGAP1 antibody 11102-1-AP	Download protocol
IHC protocol for RANGAP1 antibody 11102-1-AP	Download protocol
IF protocol for RANGAP1 antibody 11102-1-AP	Download protocol
IP protocol for RANGAP1 antibody 11102-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF,CoIP	Cell Oncol (Dordr) Hepatitis B virus core protein stabilizes RANGAP1 to upregulate KDM2A and facilitate hepatocarcinogenesis Authors - Hong-Juan You View Article
human	WB	J Proteomics mTORC1 alters the expression of glycolytic genes by regulating KPNA2 abundances. Authors - Xianwei Chen View Article
human,mouse	WB,IHC,IP	Dev Cell Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma Authors - Yan Gong View Article
human	WB,IHC,IP	Cancer Gene Ther METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1 Authors - Rui Yang KD Validated View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RANGAP1 Polyclonal antibody

RANGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 11102-1-AP

WB analysis of HEK-293 using 11102-1-AP

IP experiment of HEK-293 using 11102-1-AP

IHC staining of human gliomas using 11102-1-AP

IHC staining of human gliomas using 11102-1-AP

IF Staining of HepG2 using 11102-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Hepatitis B virus core protein stabilizes RANGAP1 to upregulate KDM2A and facilitate hepatocarcinogenesis

mTORC1 alters the expression of glycolytic genes by regulating KPNA2 abundances.

Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma

METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1