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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RanGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis of HeLa using 67146-1-Ig (same clone as 67146-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RanGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis using 67146-1-Ig (same clone as 67146-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using RanGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis of 4T1 using 67146-1-Ig (same clone as 67146-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RanGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis of HEK-293 using 67146-1-Ig (same clone as 67146-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using RanGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis of MCF-7 using 67146-1-Ig (same clone as 67146-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using RanGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis of Jurkat using 67146-1-Ig (same clone as 67146-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using RanGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis of HSC-T6 using 67146-1-Ig (same clone as 67146-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using RanGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis of NIH/3T3 using 67146-1-Ig (same clone as 67146-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RanGAP1 Monoclonal antibody (67146-1-Ig)

    IF Staining of HepG2 using 67146-1-Ig (same clone as 67146-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67146-1-Ig (RANGAP1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

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RanGAP1 Monoclonal antibody, PBS Only

RanGAP1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67146-1-PBS
Clone No.1F3A4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

1F3A4, KIAA1835, Ran GTPase-activating protein 1, SD

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67146-1-PBS targets RanGAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RanGAP1 fusion protein Ag26136 相同性解析による交差性が予測される生物種
Full Name Ran GTPase activating protein 1
Calculated molecular weight 64 kDa
Observed molecular weight 64 kDa, 82 kDa
GenBank accession numberBC014044
Gene Symbol RANGAP1
Gene ID (NCBI) 5905
RRIDAB_2882445
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP46060
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting.