• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RANGAP1 Monoclonal antibody, PBS Only

RANGAP1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F3A4

Cat no : 67146-1-PBS

Synonyms

SD, Ran GTPase-activating protein 1, KIAA1835, 1F3A4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using RANGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis of HeLa using 67146-1-Ig (same clone as 67146-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RANGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis using 67146-1-Ig (same clone as 67146-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using RANGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis of 4T1 using 67146-1-Ig (same clone as 67146-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RANGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis of HEK-293 using 67146-1-Ig (same clone as 67146-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using RANGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis of MCF-7 using 67146-1-Ig (same clone as 67146-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using RANGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis of Jurkat using 67146-1-Ig (same clone as 67146-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using RANGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis of HSC-T6 using 67146-1-Ig (same clone as 67146-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using RANGAP1 Monoclonal antibody (67146-1-Ig)

    WB analysis of NIH/3T3 using 67146-1-Ig (same clone as 67146-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RANGAP1 Monoclonal antibody (67146-1-Ig)

    IF Staining of HepG2 using 67146-1-Ig (same clone as 67146-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67146-1-Ig (RANGAP1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67146-1-PBS targets RANGAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RANGAP1 fusion protein Ag26136 相同性解析による交差性が予測される生物種
Full Name Ran GTPase activating protein 1
Calculated molecular weight 64 kDa
Observed molecular weight 64 kDa, 82 kDa
GenBank accession numberBC014044
Gene symbol RANGAP1
Gene ID (NCBI) 5905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting.