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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RANGRF Polyclonal antibody

RANGRF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10552-1-AP

Synonyms

RanGNRF, Ran-binding protein MOG1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of SGC-7901 cells using RANGRF Polyclonal antibody (10552-1-AP)

    WB analysis of SGC-7901 using 10552-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10552-1-AP (RANGRF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RANGRF Polyclonal antibody (10552-1-AP)

    WB analysis of HepG2 using 10552-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10552-1-AP (RANGRF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using RANGRF Polyclonal antibody (10552-1-AP)

    WB analysis of mouse testis using 10552-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10552-1-AP (RANGRF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using RANGRF Polyclonal antibody (10552-1-AP)

    WB analysis of mouse lung using 10552-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 10552-1-AP (RANGRF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RANGRF Polyclonal antibody (10552-1-AP)

    IHC staining of human colon cancer using 10552-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10552-1-AP (RANGRF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RANGRF Polyclonal antibody (10552-1-AP)

    IHC staining of human colon cancer using 10552-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10552-1-AP (RANGRF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RANGRF Polyclonal antibody (10552-1-AP)

    IF Staining of HepG2 using 10552-1-AP

    Immunofluorescent analysis of HepG2 cells using 10552-1-AP (RANGRF antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inSGC-7901 cells, HepG2 cells, mouse lung tissue, mouse testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

10552-1-AP targets RANGRF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RANGRF fusion protein Ag0824 相同性解析による交差性が予測される生物種
Full Name RAN guanine nucleotide release factor
Calculated molecular weight 18 kDa
Observed molecular weight 18-22 kDa
GenBank accession numberBC006486
Gene symbol RANGRF
Gene ID (NCBI) 29098
RRIDAB_2238173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RANGRF, also named as MOG1, RANGNRF, HSPC165, HSPC236 and MDS5, regulates the intracellular trafficking of RAN. In cardiac cells it regulates the cell surface localization of SCN5A.

Protocols

Product Specific Protocols
WB protocol for RANGRF antibody 10552-1-APDownload protocol
IHC protocol for RANGRF antibody 10552-1-APDownload protocol
IF protocol for RANGRF antibody 10552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Sci Transl Med

Gene therapy targeting protein trafficking regulator MOG1 in mouse models of Brugada syndrome, arrhythmias, and mild cardiomyopathy.

Authors - Gang Yu
View Article

Stem Cell Res

Generation of a homozygous RANGRF knockout hiPSC line by CRISPR/Cas9 system

Authors - Meiling Jiang
View Article