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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAP1A Polyclonal antibody

RAP1A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16336-1-AP

Synonyms

C21KG, G 22K, GTP binding protein smg p21A, KREV 1, KREV1, RAP1, RAP1A, Ras related protein Krev 1, Ras related protein Rap 1A, SMGP21

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAP1A Polyclonal antibody (16336-1-AP)

    WB analysis using 16336-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16336-1-AP (RAP1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, C6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16336-1-AP targets RAP1A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAP1A fusion protein Ag9488 相同性解析による交差性が予測される生物種
Full Name RAP1A, member of RAS oncogene family
Calculated molecular weight 184 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC014086
Gene symbol RAP1A
Gene ID (NCBI) 5906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771).

Protocols

Product Specific Protocols
WB protocol for RAP1A antibody 16336-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols