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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RAP1A Recombinant antibody (83708-1-RR)

    IF Staining of NIH/3T3 using 83708-1-RR (same clone as 83708-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RAP1A antibody (83708-1-RR, Clone: 240438G5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using RAP1A Recombinant antibody (83708-1-RR)

    FC experiment of HeLa using 83708-1-RR (same clone as 83708-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAP1A Recombinant antibody (83708-1-RR, Clone:240438G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83708-1-RR

    Affinity and Kinetic Characterization of 83708-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83708-1-RR against Human RAP1A were performed. The affinity constant is below 1 pM.

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RAP1A Recombinant antibody, PBS Only

RAP1A Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83708-1-PBS
Clone No.240438G5

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Ras-related protein Krev-1, KREV1, GTP-binding protein smg p21A, G-22K, EC:3.6.5.2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83708-1-PBS targets RAP1A in IF/ICC, FC (Intra), ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAP1A fusion protein Ag9488 相同性解析による交差性が予測される生物種
Full Name RAP1A, member of RAS oncogene family
Calculated molecular weight 184 aa, 21 kDa
GenBank accession numberBC014086
Gene Symbol RAP1A
Gene ID (NCBI) 5906
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP62834
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.