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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAP1B Monoclonal antibody (67174-1-Ig)

    WB analysis using 67174-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67174-1-Ig (RAP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using RAP1B Monoclonal antibody (67174-1-Ig)

    WB analysis of HSC-T6 using 67174-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67174-1-Ig (RAP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RAP1B Monoclonal antibody (67174-1-Ig)

    WB analysis of NIH/3T3 using 67174-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67174-1-Ig (RAP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using RAP1B Monoclonal antibody (67174-1-Ig)

    WB analysis of 4T1 using 67174-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67174-1-Ig (RAP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using RAP1B Monoclonal antibody (67174-1-Ig)

    IHC staining of human oesophagus cancer using 67174-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67174-1-Ig (RAP1B antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using RAP1B Monoclonal antibody (67174-1-Ig)

    IHC staining of human oesophagus cancer using 67174-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67174-1-Ig (RAP1B antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAP1B Monoclonal antibody (67174-1-Ig)

    IF Staining of HeLa using 67174-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAP1B antibody (67174-1-Ig, Clone: 2E7G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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RAP1B Monoclonal antibody

RAP1B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67174-1-Ig
Clone No.2E7G11

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DKFZp586H0723, GTP binding protein smg p21B, K REV, RAL1B, RAP1B, Ras related protein Rap 1b

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67174-1-Ig targets RAP1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAP1B fusion protein Ag29212 相同性解析による交差性が予測される生物種
Full Name RAP1B, member of RAS oncogene family
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC000176
Gene Symbol RAP1B
Gene ID (NCBI) 5908
RRIDAB_2882470
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61224
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAP1B antibody 67174-1-IgDownload protocol
IHC protocol for RAP1B antibody 67174-1-IgDownload protocol
IF protocol for RAP1B antibody 67174-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols