RAP1GDS1 Polyclonal antibody

RAP1GDS1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10377-1-AP

Synonyms

Exchange factor smgGDS, GDS1, RAP1GDS1, SMG GDS protein, SmgGDS


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Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10377-1-AP targets RAP1GDS1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity chicken, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAP1GDS1 fusion protein Ag0230 相同性解析による交差性が予測される生物種
Full Name RAP1, GTP-GDP dissociation stimulator 1
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC001851
Gene symbol RAP1GDS1
Gene ID (NCBI) 5910
RRIDAB_2284927
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GDS stimulates the GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins), including at least c-Ki-ras p21, smg p21A, smg p21B, rhoA p21 and rhoB p21, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. The small GTPase Rap1, which is activated by a large variety of stimuli, functions in the control of integrin-mediated cell adhesion. RAP1GDS1 (synonym: GDS1)is one of RAP1 GTP-GDP dissociation stimulators.

Protocols

Product Specific Protocols
WB protocol for RAP1GDS1 antibody 10377-1-APDownload protocol
IHC protocol for RAP1GDS1 antibody 10377-1-APDownload protocol
IP protocol for RAP1GDS1 antibody 10377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IP

Nat Genet

Mutations in RABL3 alter KRAS prenylation and are associated with hereditary pancreatic cancer.

Authors - Sahar Nissim
chickenWB,IF

Microbiol Spectr

Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.

Authors - Beibei Zhang
humanWB

Oncol Lett

SUMO4 small interfering RNA attenuates invasion and migration via the JAK2/STAT3 pathway in non-small cell lung cancer cells.

Authors - Jin-Xiao Liang
human,mouseWB

Commun Biol

Vimar/RAP1GDS1 promotes acceleration of brain aging after flies and mice reach middle age

Authors - Ying Xiong