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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAPGEF5 Polyclonal antibody

RAPGEF5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, RIP, ELISA

Conjugate

Unconjugated

Cat no : 12556-1-AP

Synonyms

GFR, KIAA0277, M Ras regulated Rap GEF, MR GEF, MRGEF, RAPGEF5, Related to Epac, REPAC

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using RAPGEF5 Polyclonal antibody (12556-1-AP)

    WB analysis of human brain using 12556-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RAPGEF5 Polyclonal antibody (12556-1-AP)

    WB analysis of human brain using 12556-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RAPGEF5 Polyclonal antibody (12556-1-AP)

    WB analysis of mouse brain using 12556-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAPGEF5 Polyclonal antibody (12556-1-AP)

    WB analysis of HeLa using 12556-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RAPGEF5 Polyclonal antibody (12556-1-AP)

    WB analysis of HEK-293 using 12556-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using RAPGEF5 Polyclonal antibody (12556-1-AP)

    WB analysis of K-562 using 12556-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inhuman brain tissue, HEK-293 cells, HeLa cells, K-562 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12556-1-AP targets RAPGEF5 in WB, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAPGEF5 fusion protein Ag3229 相同性解析による交差性が予測される生物種
Full Name Rap guanine nucleotide exchange factor (GEF) 5
Calculated molecular weight68 kDa
Observed molecular weight 68-75 kDa
GenBank accession numberBC039203
Gene symbol RAPGEF5
Gene ID (NCBI) 9771
RRIDAB_2177476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAPGEF5 antibody 12556-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,RIP

Redox Biol

CircRAPGEF5 interacts with RBFOX2 to confer ferroptosis resistance by modulating alternative splicing of TFRC in endometrial cancer

Authors - Jun Zhang
View Article