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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAPGEFL1 Polyclonal antibody

RAPGEFL1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21746-1-AP

Synonyms

Link GEFII, RAPGEFL1

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using RAPGEFL1 Polyclonal antibody (21746-1-AP)

    WB analysis of human brain using 21746-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21746-1-AP (RAPGEFL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using RAPGEFL1 Polyclonal antibody (21746-1-AP)

    WB analysis of mouse kidney using 21746-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21746-1-AP (RAPGEFL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RAPGEFL1 Polyclonal antibody (21746-1-AP)

    IF Staining of HepG2 using 21746-1-AP

    Immunofluorescent analysis of HepG2 cells, using RAPGEFL1 antibody 21746-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inhuman brain tissue, mouse kidney tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21746-1-AP is RAPGEFL1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAPGEFL1 fusion protein Ag16506 相同性解析による交差性が予測される生物種
Full Name Rap guanine nucleotide exchange factor (GEF)-like 1
Calculated molecular weight 662 aa, 73 kDa
Observed molecular weight 73 kDa, 59 kDa
GenBank accession numberBC115374
Gene symbol RAPGEFL1
Gene ID (NCBI) 51195
RRIDAB_10733650
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAPGEFL1 antibody 21746-1-APDownload protocol
IF protocol for RAPGEFL1 antibody 21746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols