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Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using RAPH1 Polyclonal antibody (25750-1-AP)

    WB analysis of SKOV-3 using 25750-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25750-1-AP (RAPH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RAPH1 Polyclonal antibody (25750-1-AP)

    IF Staining of HepG2 using 25750-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RAPH1 antibody (25750-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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RAPH1 Polyclonal antibody

RAPH1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25750-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Lamellipodin, KIAA1681, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein, ALS2CR9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSKOV-3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25750-1-AP targets RAPH1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAPH1 fusion protein Ag22705 相同性解析による交差性が予測される生物種
Full Name Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
Calculated molecular weight 1250 aa, 135 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC156922
Gene Symbol RAPH1
Gene ID (NCBI) 65059
RRIDAB_2880223
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ70E73
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAPH1 encodes the lamellipodin protein (Lpd), which binds to Ena and VASPs to assist in actin cytoskeleton assembly; in this way, the gene plays an important role in cellular motility and lamelipodial protrusion. This protein has 9 isoforms producing different molecular weights of 135 kDa, 67 kDa, 70 kDa, 73 kDa.

Protocols

Product Specific Protocols
WB protocol for RAPH1 antibody 25750-1-APDownload protocol
IF protocol for RAPH1 antibody 25750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols