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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAPSN Polyclonal antibody

RAPSN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20453-1-AP

Synonyms

43 kDa postsynaptic protein, CMS1D, CMS1E, RAPSN, RAPsyn, RING finger protein 205, RNF205

Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using RAPSN Polyclonal antibody (20453-1-AP)

    WB analysis of U-937 using 20453-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RAPSN Polyclonal antibody (20453-1-AP)

    WB analysis of NIH/3T3 using 20453-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RAPSN Polyclonal antibody (20453-1-AP)

    WB analysis of NIH/3T3 using 20453-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using RAPSN Polyclonal antibody (20453-1-AP)

    WB analysis of U-937 using 20453-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using RAPSN Polyclonal antibody (20453-1-AP)

    WB analysis of Y79 using 20453-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using RAPSN Polyclonal antibody (20453-1-AP)

    WB analysis of U-937 using 20453-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using RAPSN Polyclonal antibody (20453-1-AP)

    WB analysis of U-937 using 20453-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RAPSN Polyclonal antibody (20453-1-AP)

    WB analysis of NIH/3T3 using 20453-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RAPSN Polyclonal antibody (20453-1-AP)

    WB analysis of NIH/3T3 using 20453-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using RAPSN Polyclonal antibody (20453-1-AP)

    WB analysis of Y79 using 20453-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using RAPSN Polyclonal antibody (20453-1-AP)

    WB analysis of Y79 using 20453-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 20453-1-AP (RAPSN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inU-937 cells, NIH/3T3 cells, Y79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 20453-1-AP is RAPSN Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAPSN fusion protein Ag13896 相同性解析による交差性が予測される生物種
Full Name receptor-associated protein of the synapse
Calculated molecular weight 412 aa, 46 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC004196
Gene symbol RAPSN
Gene ID (NCBI) 5913
RRIDAB_2878689
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAPSN antibody 20453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Appl Physiol (1985)

Plasticity changes in neuromuscular junction morphology and related regulatory proteins in the hibernating ground squirrel

Authors - Yue He
View Article