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  • Featured Product
  • KD/KO Validated

RARB Polyclonal antibody

RARB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14013-1-AP

Synonyms

Retinoic acid receptor beta, RAR-epsilon, RAR-beta, RAR beta, NR1B2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RARB Polyclonal antibody (14013-1-AP)

    WB analysis using 14013-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14013-1-AP (RARB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of mouse colon using 14013-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of mouse colon using 14013-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of mouse bladder using 14013-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of human pancreas cancer using 14013-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of human renal cell carcinoma using 14013-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of mouse brain using 14013-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat bladder tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of rat bladder using 14013-1-AP

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using RARB Polyclonal antibody (14013-1-AP)

    IHC staining of rat brain using 14013-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RARB Polyclonal antibody (14013-1-AP)

    IF Staining of MCF-7 using 14013-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RARB antibody (14013-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inSp2/0 cells
Positive IHC detected inmouse colon tissue, mouse bladder tissue, human pancreas cancer tissue, human renal cell carcinoma tissue, mouse brain tissue, rat bladder tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14013-1-AP targets RARB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RARB fusion protein Ag5119 相同性解析による交差性が予測される生物種
Full Name retinoic acid receptor, beta
Calculated molecular weight 50 kDa
Observed molecular weight37-51 kDa
GenBank accession numberBC060794
Gene symbol RARB
Gene ID (NCBI) 5915
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Retinoic acid receptors (RARs) are important mediators of retinoid signaling in morphogenesis, development, and cell differentiation. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes (PMID:12554770). They can bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, they act mainly as activators of gene expression due to weak binding to corepressors. RARB is one isotypes of RARs (PMID:12834868).

Protocols

Product Specific Protocols
WB protocol for RARB antibody 14013-1-APDownload protocol
IHC protocol for RARB antibody 14013-1-APDownload protocol
IF protocol for RARB antibody 14013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Antiviral Res

Retinoic acid receptor β, a potential therapeutic target in the inhibition of adenovirus replication.

Authors - Xiaolong Wang
  • KD Validated
View Article
human,mouseWB

iScience

Alphaherpesvirus manipulates retinoic acid metabolism for optimal replication

Authors - Shengli Ming
View Article