Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HeLa using 27344-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 27344-1-AP (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 27344-1-AP
WB result of RARS antibody (27344-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RARS transfected HeLa cells.
WB analysis of U-251 using 27344-1-AP
U-251 cells were subjected to SDS PAGE followed by western blot with 27344-1-AP (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 27344-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 27344-1-AP (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 27344-1-AP
IP result of anti-RARS (IP:27344-1-AP, 4ug; Detection:27344-1-AP 1:500) with HeLa cells lysate 3200 ug.
IHC staining of human heart using 27344-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human heart using 27344-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human breast cancer using 27344-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 27344-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

RARS Polyclonal antibody

RARS Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 27344-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Synonyms

Arginine tRNA ligase, arginyl tRNA synthetase, ArgRS, DALRD1, RARS

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A431 cells, U-251 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human heart tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 3 publications below

Product Information

27344-1-AP targets RARS in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RARS fusion protein Ag26390 相同性解析による交差性が予測される生物種
Full Name	arginyl-tRNA synthetase
Calculated molecular weight	75 kDa
Observed molecular weight	67 kDa
GenBank accession number	BC000528
Gene Symbol	RARS
Gene ID (NCBI)	5917
RRID	AB_2880849
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P54136
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RARS, also named ArgRS (arginyl-tRNA synthetase), belongs to the class-I aminoacyl-tRNA synthetase family which catalyze the aminoacylation of tRNA by their cognate amino acid. In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC) (PMID:25288775). RARS has two isoforms with the molecular weight of 75 and 67 kDa.

Protocols

Product Specific Protocols
WB protocol for RARS antibody 27344-1-AP	Download protocol
IHC protocol for RARS antibody 27344-1-AP	Download protocol
IP protocol for RARS antibody 27344-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell Arginine deprivation enriches lung cancer proteomes with cysteine by inducing arginine-to-cysteine substitutants Authors - Chao Yang KD Validated View Article
human	WB	bioRxiv Arginine limitation causes a directed DNA sequence evolution response in colorectal cancer cells Authors - Dennis J Hsu KD Validated View Article
human	WB	Sci Adv Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells Authors - Dennis J Hsu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 27344-1-AP

WB analysis of HeLa using 27344-1-AP

WB analysis of U-251 using 27344-1-AP

WB analysis of A431 using 27344-1-AP

IP experiment of HeLa using 27344-1-AP

IHC staining of human heart using 27344-1-AP