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  • Featured Product
  • KD/KO Validated

RARS Monoclonal antibody, PBS Only

RARS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C8G4

Cat no : 66815-1-PBS

Synonyms

Arginine tRNA ligase, arginyl tRNA synthetase, ArgRS, DALRD1, RARS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RARS Monoclonal antibody (66815-1-Ig)

    WB analysis using 66815-1-Ig (same clone as 66815-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66815-1-Ig (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66815-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RARS Monoclonal antibody (66815-1-Ig)

    WB analysis using 66815-1-Ig (same clone as 66815-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66815-1-Ig (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66815-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RARS Monoclonal antibody (66815-1-Ig)

    WB analysis of HeLa using 66815-1-Ig (same clone as 66815-1-PBS)

    WB result of RARS antibody (66815-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RARS transfected HeLa cells. This data was developed using the same antibody clone with 66815-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RARS Monoclonal antibody (66815-1-Ig)

    IF Staining of HeLa using 66815-1-Ig (same clone as 66815-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RARS antibody (66815-1-Ig, Clone: 1C8G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66815-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66815-1-PBS targets RARS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RARS fusion protein Ag26399 相同性解析による交差性が予測される生物種
Full Name arginyl-tRNA synthetase
Calculated molecular weight 75 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC000528
Gene symbol RARS
Gene ID (NCBI) 5917
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RARS, also named ArgRS (arginyl-tRNA synthetase), belongs to the class-I aminoacyl-tRNA synthetase family which catalyze the aminoacylation of tRNA by their cognate amino acid. In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC) (PMID:25288775). RARS has two isoforms with the molecular weight of 75 and 67 kDa.