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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RASGAP Polyclonal antibody

RASGAP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12935-1-AP

Synonyms

RASA1, Ras p21 protein activator, Ras GTPase-activating protein 1, p120RASGAP, p120GAP

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RASGAP Polyclonal antibody (12935-1-AP)

    WB analysis using 12935-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12935-1-AP (RASGAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using RASGAP Polyclonal antibody (12935-1-AP)

    WB analysis of mouse testis using 12935-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12935-1-AP (RASGAP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using RASGAP Polyclonal antibody (12935-1-AP)

    IP experiment of mouse testis using 12935-1-AP

    IP result of anti-RASGAP (IP:12935-1-AP, 4ug; Detection:12935-1-AP 1:4000) with mouse testis tissue lysate 1120 ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using RASGAP Polyclonal antibody (12935-1-AP)

    IHC staining of human testis using 12935-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RASGAP Polyclonal antibody (12935-1-AP)

    IHC staining of human testis using 12935-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RASGAP Polyclonal antibody (12935-1-AP)

    IHC staining of human breast cancer using 12935-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RASGAP Polyclonal antibody (12935-1-AP)

    IHC staining of human breast cancer using 12935-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RASGAP Polyclonal antibody (12935-1-AP)

    IF Staining of HeLa using 12935-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RASGAP antibody (12935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12935-1-AP targets RASGAP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RASGAP fusion protein Ag3906 相同性解析による交差性が予測される生物種
Full Name RAS p21 protein activator (GTPase activating protein) 1
Calculated molecular weight 1047 aa, 116 kDa
Observed molecular weight 120 kDa, 95 kDa
GenBank accession numberBC033015
Gene symbol RASA1
Gene ID (NCBI) 5921
RRIDAB_2175906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RASGAP antibody 12935-1-APDownload protocol
IHC protocol for RASGAP antibody 12935-1-APDownload protocol
IF protocol for RASGAP antibody 12935-1-APDownload protocol
IP protocol for RASGAP antibody 12935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Biochem Biophys Res Commun

miR-335 is involved in the rat epididymal development by targeting the mRNA of RASA1.

Authors - Wang Junfeng J
View Article
humanWB

Int J Clin Exp Pathol

Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.

Authors - Xiaojuan Ye
View Article