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Validation Data Gallery

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  • Western Blot (WB) analysis of 3T3-L1 cells using RASA2/3 Polyclonal antibody (27835-1-AP)

    WB analysis of 3T3-L1 using 27835-1-AP

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 27835-1-AP (RASA2/3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RASA2/3 Polyclonal antibody (27835-1-AP)

    WB analysis of HeLa using 27835-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27835-1-AP (RASA2/3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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RASA2/3 Polyclonal antibody

RASA2/3 Polyclonal Antibody for WB, ELISA
Cat No. 27835-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Synonyms

GAP1(IP4BP), GAP1IP4BP, GAPIII, Ins P4 binding protein, RAS p21 protein activator 3, RASA2/3, RASA3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in3T3-L1 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

27835-1-AP targets RASA2/3 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RASA2/3 fusion protein Ag27152 相同性解析による交差性が予測される生物種
Full Name RAS p21 protein activator 3
Calculated molecular weight 834 aa, 96 kDa
Observed molecular weight 96 kDa
GenBank accession numberBC038456
Gene Symbol RASA3
Gene ID (NCBI) 22821
RRIDAB_2880988
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14644
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rasa3 is a GTPase activating protein of the GAP1 family which targets R-Ras and Rap1. While R-Ras has been extensively studied due to its involvement in cancer, Rap1 has recently attracted a lot of attention due to its central role in development and morphogenesis of higher organisms, especially in the cardiovasculature. This antibody can react with RASA2 and RASA3.

Protocols

Product Specific Protocols
WB protocol for RASA2/3 antibody 27835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Arterioscler Thromb Vasc Biol

Lipid Receptor GPR31 (G-Protein-Coupled Receptor 31) Regulates Platelet Reactivity and Thrombosis Without Affecting Hemostasis.

Authors - Layla Van Doren
View Article
mouseWB

Front Immunol

The Rac-GEF Tiam1 controls integrin-dependent neutrophil responses

Authors - Kirsti Hornigold
View Article