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  • Featured Product
  • KD/KO Validated

RASEF Polyclonal antibody, PBS Only

RASEF Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11569-1-PBS

Synonyms

Ras-related protein Rab-45, Ras related protein Rab 45, Ras and EF-hand domain-containing protein, RAB45

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using RASEF Polyclonal antibody (11569-1-AP)

    WB analysis of mouse testis using 11569-1-AP (same clone as 11569-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11569-1-AP (RASEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11569-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using RASEF Polyclonal antibody (11569-1-AP)

    WB analysis of mouse heart using 11569-1-AP (same clone as 11569-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11569-1-AP (RASEF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11569-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RASEF Polyclonal antibody (11569-1-AP)

    IHC staining of mouse brain using 11569-1-AP (same clone as 11569-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11569-1-AP (RASEF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11569-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RASEF Polyclonal antibody (11569-1-AP)

    IHC staining of mouse brain using 11569-1-AP (same clone as 11569-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11569-1-AP (RASEF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11569-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11569-1-PBS targets RASEF in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RASEF fusion protein Ag2149 相同性解析による交差性が予測される生物種
Full Name RAS and EF-hand domain containing
Calculated molecular weight 740 aa, 83 kDa
Observed molecular weight 83 kDa
GenBank accession numberBC023566
Gene symbol RASEF
Gene ID (NCBI) 158158
RRIDAB_2238112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.