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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RASEF Monoclonal antibody, PBS Only

RASEF Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C2B2

Cat no : 67159-1-PBS

Synonyms

FLJ31614, RAB45, Ras related protein Rab 45, RASEF

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of LNCaP cells using RASEF Monoclonal antibody (67159-1-Ig)

    WB analysis of LNCaP using 67159-1-Ig (same clone as 67159-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67159-1-Ig (RASEF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of mouse pancreas using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of mouse pancreas using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human ovary tumor using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human ovary tumor using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human pancreas cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human pancreas cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RASEF Monoclonal antibody (67159-1-Ig)

    IF Staining of A431 using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RASEF antibody (67159-1-Ig, Clone: 1C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67159-1-PBS targets RASEF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RASEF fusion protein Ag2149 相同性解析による交差性が予測される生物種
Full Name RAS and EF-hand domain containing
Calculated molecular weight 740 aa, 83 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC023566
Gene symbol RASEF
Gene ID (NCBI) 158158
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The RASEF (RAS and EF-hand domain-containing protein), a member of Rab GTPase protein family, contains a Rab GTPase domain in the C-terminal region, a coiled coil motif in an internal region as well as 2 EF hand domains which were of importance for binding to calcium ions in the N-terminus.