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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RASGEF1B Polyclonal antibody

RASGEF1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13617-1-AP

Synonyms

GPI gamma 4, GPIG4, RASGEF1B

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using RASGEF1B Polyclonal antibody (13617-1-AP)

    WB analysis of human brain using 13617-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13617-1-AP (RASGEF1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using RASGEF1B Polyclonal antibody (13617-1-AP)

    WB analysis of mouse lung using 13617-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13617-1-AP (RASGEF1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using RASGEF1B Polyclonal antibody (13617-1-AP)

    WB analysis of mouse ovary using 13617-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13617-1-AP (RASGEF1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RASGEF1B Polyclonal antibody (13617-1-AP)

    IHC staining of human stomach using 13617-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 13617-1-AP (RASGEF1B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using RASGEF1B Polyclonal antibody (13617-1-AP)

    IHC staining of human stomach using 13617-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 13617-1-AP (RASGEF1B antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inhuman brain tissue, mouse lung tissue, mouse ovary tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13617-1-AP targets RASGEF1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RASGEF1B fusion protein Ag4525 相同性解析による交差性が予測される生物種
Full Name RasGEF domain family, member 1B
Calculated molecular weight 55 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC036784
Gene symbol RASGEF1B
Gene ID (NCBI) 153020
RRIDAB_10644147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RASGEF1B is a guanine-nucleotide exchange factor, whose expression is induced inmacrophages on stimulation by TLR2 and TLR4 agonists. It interacts with Ras family proteins. It also interacts with CCDC124 during cytokinesis.

Protocols

Product Specific Protocols
WB protocol for RASGEF1B antibody 13617-1-APDownload protocol
IHC protocol for RASGEF1B antibody 13617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols