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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RASGRP1 Polyclonal antibody

RASGRP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26997-1-AP

Synonyms

Ras guanyl releasing protein, hRasGRP1, CalDAG-GEFII, CALDAG GEFII, CALDAG GEFI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RASGRP1 Polyclonal antibody (26997-1-AP)

    WB analysis using 26997-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26997-1-AP (RASGRP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RASGRP1 Polyclonal antibody (26997-1-AP)

    IF Staining of HepG2 using 26997-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RASGRP1 antibody (26997-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26997-1-AP targets RASGRP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RASGRP1 fusion protein Ag25725 相同性解析による交差性が予測される生物種
Full Name RAS guanyl releasing protein 1 (calcium and DAG-regulated)
Calculated molecular weight 797 aa, 90 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC109297
Gene symbol RASGRP1
Gene ID (NCBI) 10125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RASGRP1 (RAS guanyl-releasing protein 1) is a Ras guanine nucleotide exchange factor, and an essential regulator of lymphocyte receptor signaling (PMID: 33065764). RasGRP1 is a bifunctional regulator that promotes acute inflammation and inhibits inflammation-associated cancer (PMID: 36385095). RasGRP1, a downstream target gene of VEGF, regulates the development, homeostasis, and differentiation of T cells (PMID: 37429143).

Protocols

Product Specific Protocols
WB protocol for RASGRP1 antibody 26997-1-APDownload protocol
IF protocol for RASGRP1 antibody 26997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols